Submit your manuscripts at http://www.hindawi.com
High quality Rabbit Monoclonal Antibodies with high affinity Antibody Binding Affinity (KD) KD [M] 1.E-13 1. E-13 1.E-12 1.E-11
Average KD value of RabMAbs
1 x 10-10 M
1.E-10 1.E-09
Average KD value of Mouse MAbs
1 x 10-8 M
1.E-08 0
100
200
300
400
500
600
RabMAbs*
Mouse*
Antibody kinetic analysis: Binding affinity constant (KD) * Partial literature survey ** In-house KD measurements
KD
We have systematically measured the KD (the equilibrium dissociation constant between the antibody and its antigen), in over 800 Rabbit Monoclonal Antibodies (RabMAbs). Based on the comparison with published literature values for mouse monoclonal antibodies, RabMAbs appear to be on average 1-2 orders of magnitude higher affinity.
Discover more at abcam.com/KD
RabMAbs
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Gibson Assembly® Cloning Kit
Making ends meet is now quicker and easier than ever before, with the Gibson Assembly Cloning Kit from NEB.
Visit NEBGibson.com to view the latest tutorials and to try our primer design tool. IN-FUSION® is a registered trademark of Clontech Laboratories, Inc. GENEART® is a registered trademark of Life Technologies, Inc. GIBSON ASSEMBLY® is a registered trademark of Synthetic Genomics, Inc.
Gibson Assembly Cloning Kit provides robust transformation efficiencies 3000 Number of recombinant colonies (x103)
New England Biolabs has revolutionized your laboratory’s standard cloning methodology. The Gibson Assembly Cloning Kit combines the power of the Gibson Assembly Master Mix with NEB 5-alpha Competent E. coli, enabling fragment assembly and transformation in just under two hours. Save time, without sacrificing efficiency.
Gibson Assembly In-Fusion® GeneArt®
2500 2000 1500 1000 500
20 10 0
1 kb
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5 kb
Assembly reactions containing 25 ng of linear pUC19 vector and 0.04 pmol of each fragment were performed following individual suppliers’ recommended protocols and using the competent cells provided with the kit. The total number of recombinant colonies was calculated per 25 ng of linear pUC19 vector added to the assembly reaction. Some components of this product are manufactured by New England Biolabs, Inc. under license from Synthetic Genomics, Inc.
“ cancer metabolism research WE’RE BRINGING A NEW PERSPECTIVE TO
FIRST WE MADE IT POSSIBLE – NOW WE’VE MADE IT EASY. XF technology provides the easiest and most comprehensive assessment of cancer cell metabolism, measuring glucose and glutamine metabolism, and fatty acid oxidation of cancer cells in a microplate, in real-time!
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The Seahorse XFe Extracellular Flux Analyzer Measurements of cellular glycolysis are essential to understanding cancer, cer, immune response, stem cell diferentiation, aging, and cardiovascular and neurodegenerative odegenerative diseases. e The XF Analyzer and XF Glycolysis Stress Test Kit make it easy to measure the three key parameters of cellular glycolysis in a microplate: glycolysis, glycolytic capacity, and glycolytic reserve, revealing critical information not evident in mitochondrial respiration measurements alone.
See what’s possible. Scan this QR code to view videos and see what the XF Analyzer can achieve. Visit www.seahorsebio.com/science for more information!
Confidence. Inspired by Results.
Alan Alfano Cancer Researcher, University of Maryland School of Medicine, Baltimore, MD
“In cancer research, achieving uniform results with transfection is often a big hurdle. X-tremeGENE is by far the best transfection reagent I’ve ever used. It’s nice to know that with all the potential issues I might encounter in my research, transfection isn’t one of them.” X-tremeGENE Reagents. Transfect with confidence. See more of Alan‘s story at www.x-tremegene.roche.com
For life science research only. Not for use in diagnostic procedures. X-TREMEGENE is a trademark of Roche.
Roche Diagnostics Corporation Roche Applied Science Indianapolis, Indiana © 2013 Roche Diagnostics. All rights reserved.
I N T E R N AT ION A L SCIENCE & ENGINEERING
VISUALIZATION C H A L L E N G E
Deadline Approaching ENTRY DEADLINE: SEPTEMBER 30, 2013 sc ienc e & e ng ineering’s m ost powe r fu l statem ents are not m ad e f rom word s alo ne
You are invited to submit an entry to this year’s International Science
Award Cate gories
& Engineering Visualization Challenge, cosponsored by the National
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Science Foundation and AAA’s journal Science. Entry categories include: Photography, Illustration, Posters and Graphics, Games & Apps, and Video. Winning entries will be published in a special section of a February 2014 print issue of Science and ScienceMag.org, and NSF’s web site. ENTRY DEADLINE: SEPTEMBER 30, 2013 For entry forms, rules, and more information, go to:
www.nsf.gov/news/scivis
Photo grap hy Illus tration Pos ter s & G rap hic s V ide o G am es & Apps
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New accessory: Eppendorf ThermoTop®
Multiple Talents Eppendorf ThermoMixer™ and Eppendorf ThermoStat™ The new generation of Eppendorf Tem> 2DMix-Control: perfect mixing results perature Control and Mixing Instruments in all vessel formats ofer you more than what meets the eye. > Eppendorf QuickRelease™: ergonomic Not only do they provide the versatility and accurate temperature control you come to expect, they now also ofer you superior mixing performance, outstanding ergonomic operation and a unique ThermoTop.
block exchange in just 2 seconds > Intuitive operation: pre-optimized program and temperature keys > Eppendorf ThermoTop prevents condensate formation for more accurate results.
www.eppendorf.com/thermomixer-c Eppendorf®, the Eppendorf logo and Eppendorf ThermoTop® are registered Trademarks of Eppendorf AG, Germany. Eppendorf ThermoMixer™, Eppendorf SmartBlocks™, Eppendorf ThermoStat™ and Eppendorf QuickRelease™ are Trademarks of Eppendorf AG. All rights reserved, including images and graphics. Copyright © 2013 by Eppendorf AG.
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Electronically submit your new product description or product literature information! Go to www.sciencemag.org/products/newproducts.dtl for more information. Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availability of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.
www.sciencemag.org/products
SCIENCE
VOL 341
6 SEPTEMBER 2013
1127
AAAS 2014
ANNUAL MEETING
13 - 17 F E B R U A R Y • C H I C A G O
MEETI NG GLOBAL CHALLENGES:
DISCOVERY AND INNOVATION
Plenary Speakers
Alan Alda
Steven Chu
Visiting Professor of Journalism Stony Brook University
Professor of Physics and Molecular and Cellular Physiology Stanford University
Getting Beyond a Blind Date with Science
How Discovery and Innovation Can Meet Our Energy Challenge
Susan Lindquist
John A. Rogers
Professor of Biology Massachusetts Institute of Technology
Swanlund Chair and Professor of Materials Science and Engineering University of Illinois, UrbanaChampaign
From Yeast Cells to Patient Neurons: A Powerful Discovery Platform for Parkinson’s and Alzheimer’s Diseases
Registration and housing are now open. www.aaas.org/meetings
Stretchy Electronics that Dissolve in Your Body
You’ve carefully designed your experiment.
Taylor Ngo started at CST 8 years ago and is currently in the Molecular Biology group.
Does your antibody measure up? We validate all our antibodies in-house. If it’s not specific, it doesn’t ship.
kDa 200
kDa 200
140
140
100 80
100 80
A
TFEB
60
CST APPROVED
REJECTED
Clean Band Strong Signal
Extra Band Weak Signal
50
B
TFEB IgG
60 50 40
40 30
30
20
20 –
+
Torin1
–
+
+
IP
TFEB Antibody #4240: (A) WB analysis of Raji cell extracts, untreated (-) or Torin1-treated, using #4240 (Torin1 treatment induces dephosphorylation). (B) IP of TFEB from COLO 205 cells using #4240 (lane 2) or Normal Rabbit IgG #2729 (lane 3). Lane 1 is 10% input. WB analysis of various cell extracts using two development samples at 1:1000 dilution. GAPDH (D16H11) XP® Rabbit mAb #5174 was used as a loading control.
Please visit our website to request a copy of our new white paper – A Guide to Successful Western Blotting.
www.cellsignal.com/successfulWB © 2013 Cell Signaling Technology, Inc. Cell Signaling Technology® and XP® are trademarks of Cell Signaling Technology, Inc.
