Efficient Cell-Free DNA Recovery Quick-cfDNA™ Serum & Plasma Kit (Serum, plasma, saliva, cerebrospinal fluid, and amniotic fluid) • High Processing Volume: Purify ≤ 10 ml of serum or plasma and elute with 35 µl. • Highest Yields: Consistently purify > 30% more cfDNA.
• Ultra-Pure: Ready for qPCR, Next-Gen Sequencing, etc.
Highest Yields 800
B
Zymo Research
700 600
14
Zymo Research
12
Supplier Q Total DNA yield (ng)
Cell free DNA yield (pg/µL)
A
500 400 300 200 100
Supplier Q
10 8 6 4 2
0 0
1
2
3
4
5
0
Plasma Volume (ml)
1
2
3
Plasma Volume (ml)
4
5
The Quick-cfDNA™ Serum & Plasma Kit recovers more cell-free DNA than a comparable Supplier Q kit. The DNA recovered is linearly proportional to the sample input volume when compared with Supplier Q. (A) Concentration of the smallest nucleosomal fragment DNA (N1, ~180 bp) was determined using the Agilent 2100 Bioanalyzer® system. (B) Total DNA recovery was quantified using the Zymo Research Femto™ Human DNA Quantification Kit on an Applied Biosystems® 7500 Real-Time PCR System.
Linear and Efficient Recovery of Cell-Free DNA 1 ml
5 ml
Versatile Sample Compatibility Maternal plasma
10 ml
[bp]
Amniotic fluid
Cerebrospinal fluid
[bp]
1,500
48,500 15,000 7,000 4,000 4,000 3,000 2,500 2,000 1,500 1,200 900
1,000 700 500 400 300 200
600 400
100
250 100
36yr F plasma Cell-free DNA recovery scales proportionally with sample input using the Quick-cfDNA™ Serum & Plasma Kit. Cell-free DNA was isolated in duplicate from three healthy female donors, and visualized using the Agilent 2200 Tapestation® system.
Total DNA, including both high and low molecular weight species, was purified in duplicate from human maternal plasma, amniotic fluid, and cerebrospinal fluid. DNA was visualized using the Agilent 2200 Tapestation® system.
[email protected] Product
Cat. No.
Size
www.zymoresearch.com
Quick-cfDNA™ Serum & Plasma Kit
D4076
50 preps
Toll Free: (888) 882-9682 Cat. No. DS1304
Efficient Cell-Free DNA and RNA Recovery Quick-cfRNA™ Serum & Plasma Kit Quick-cfDNA/cfRNA™ Serum & Plasma Kit (Serum, plasma, saliva, cerebrospinal fluid, and amniotic fluid) • Quick & Easy: Simple spin-column based isolation. No phenol/chloroform or precipitation.
• Highest Yields: Efficient isolation of total cell-free RNA. Recover up to 515 times more microRNA. • Ultra-Pure: Ready for RT-qPCR, Next-Gen Sequencing, nCounter®, etc.
Highest Recovery of Cell-Free miRNA
Copy number, hsa-miR-16-5p
1.0E+10
8.0E+9
6.0E+9
4.0E+9
2.0E+9
Zymo Research
Q 3. A C 0 m NA l( m Kit ax )
A C 1. NA 0 m Ki l t
Q
m 0. iR 6 A m dv l( K m it ax )
iR 0. Ad 2 v m Ki l t
m
A
Q
ui c 3. k-cf 0 D m N l( A m /R ax N ) A
-c 1. fDN 0 m A/R l N
ck ui Q
Q
ui
ck
-c 0. fDN 5 m A/R l N
A
0.0E+9
Supplier Q
Cell-free RNA recovery scales proportionally with sample input using the Quick-cfDNA/cfRNA™ Serum & Plasma Kit. Cell-free RNA yields from the same plasma donor (61y-F) show linear and efficient recovery of plasma microRNA (hsa-miR-16-5p) when analyzed by RT-qPCR. High Sensitivity D1000 ScreenTape®
Ultra-Pure & Ready for RT-qPCR
Page 1
Compatible with Various Biological Fluid
Filename: 11292017 cfRNA kit; other sample types testing (AF, CSF, used media).HSD1000
[bp] 1500
104
1000 700 500 400 300
103
RFU
200 100
10
2
50
Zymo Research (1 mL)
25
Supplier M (0.9 mL)
101
20
25
Cycles
30
35
Default image (Contrast 113%) 1 ml
Sample Info
The Quick-cfRNA Serum & Plasma Kit results in high purity and recovery of plasma microRNA (hsa-miR-16-5p) based on RT-qPCR analysis. ™
3 ml
1 ml
AF
3 ml
1 ml
CSF
Well
Conc. [pg/µl]
A1
2540
A2 B2 C2 D2 E2 F2
57.5 375 607 817 768 1.11
Media Sample Description
Ladder
3 ml
Alert
Observations
Issue with ladder peak detection (too many peaks detected); Ladder
Amniotic fluid (AF),B1cerebrospinal 249 fluid (CSF), or spent HeLa cell culture media C1 245 ™ (Media) were used to D1 isolate cell-free 480 nucleic acids using Quick-cfDNA/cfRNA E1 363 Serum & Plasma Kit. F1 Endogenous cell-free DNA from each sample type was G1 ® visualized using theH1 Agilent 2200 Tapestation system. 37.6
[email protected]
Product
Cat. No.
Size
Quick-cfRNA™ Serum & Plasma Kit
R1059
50 preps
www.zymoresearch.com
Quick-cfDNA/cfRNA™ Serum & Plasma Kit
R1072
50 preps
Toll Free: (888) 882-9682 Cat. No. DS1304